Analyse the extracted Beta Carrotene by Spectrophotometer

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 Analysis by Spectrophotometer

Beta carrotene was found in hexane layer. Now we check the beta carrotene is present in hexane or not by spectrophotometer. Take small quantity of this solution in cuvette and put in spectrophotometer and set the filter. Now set the transmittance of spectrophotometer 100% so that it contains 0% absorbance so that it doesn't interfer in absorbance of beta carrotene. Now switch on spectrophotometer and note down the absorbance at different value of wavelength  and at one value absorbance is maximum it is lambda max. At 450nm absorbance is maximum of this solution, so it indicates solution contains beta carrotene. Now take this solution and put on steam bath for 5 minutes  and after that crude beta carrotene obtained and melting point was noted down. 

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