Extraction Of Beta Carotene from carrot

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REQUIREMENTS:-

carrot,methanol,hexane,mortar pistle,waterbath,separatory funnel,spetrophotometer.


PROCEDURE:-

1.CARROT WAS TAKEN AND CRUSHED WITH THE HELP OF MORTOR AND PISTLE.
2.NOW CARROT WAS DISSOLVED IN 250 mL OF METHANOL AND COVER WITH ALUMINIUM FOIL.
3.NOW BEAKER WAS PUT ON WATERBATH FOR ONE HOUR WITH CONSTANT STIRRING.
4.NOW REMOVE FROM WATERBATH  AND COOL DOWN AT ROOM TEMPEATURE  AND FILTER.
5.NOW TAKE 10mL OF HEXANE AND 10mL OF FILTRATE IN A SEPARATORY FUNNEL AND SHAKE PROPERLY.
6.THEN TWO LAYERS SEPARATE OUT .LOWER LAYER IS OF METHANOL AND UPPER LAYER IS OF HEXANE CONTAIN BETA CARROTENE.
7.NOW TAKE UPPER LAYER  AND ENALYSE THIS LAYER. 


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